Journal: Oncotarget
Article Title: DPP4/CD26 overexpression in urothelial carcinoma confers an independent prognostic impact and correlates with intrinsic biological aggressiveness
doi: 10.18632/oncotarget.13820
Figure Lengend Snippet: ( A ) Endogenous DPP4 mRNA levels were measured by using quantitative RT-PCR (right panel) and western blotting assays (left panel). Compared with non-tumorigenic urothelial primary cell HUC, two UC cells, J82 and RTCC-1, show high DPP4 mRNA and protein expression levels. ( B ) To explore biological functions of DPP4 in vitro, DPP4 knockdown is conducted by using short-hairpin RNA which successfully deplete DPP4 transcript level in J82 (right panel) and RTCC-1 (left panel) cells. ( C ) Depletion of DPP4 expression results in a significantly decreased cell viability (upper panel), migration (middle panel), and invasion (lower panel) in J82 and RTCC-1 cells. The quantified results are presented as means ± sd. Error bars indicate the standard error of the mean. Data represent mean values of three independent experiments. (*P < 0.05).
Article Snippet: A normal urothelial cell primary cell, HUC (ScienCell Research Laboratories, San Diego, CA), was used as a control.
Techniques: Quantitative RT-PCR, Western Blot, Expressing, In Vitro, Knockdown, shRNA, Migration