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ScienCell primary normal huc
Primary Normal Huc, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+normal+huc/pm29228614-154-21-30?v=ScienCell
Average 90 stars, based on 1 article reviews
primary normal huc - by Bioz Stars, 2026-07
90/100 stars

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ScienCell normal urothelial cell primary cell huc
( A ) Endogenous DPP4 mRNA levels were measured by using quantitative RT-PCR (right panel) and western blotting assays (left panel). Compared with <t>non-tumorigenic</t> <t>urothelial</t> primary cell <t>HUC,</t> two UC cells, J82 and RTCC-1, show high DPP4 mRNA and protein expression levels. ( B ) To explore biological functions of DPP4 in vitro, DPP4 knockdown is conducted by using short-hairpin RNA which successfully deplete DPP4 transcript level in J82 (right panel) and RTCC-1 (left panel) cells. ( C ) Depletion of DPP4 expression results in a significantly decreased cell viability (upper panel), migration (middle panel), and invasion (lower panel) in J82 and RTCC-1 cells. The quantified results are presented as means ± sd. Error bars indicate the standard error of the mean. Data represent mean values of three independent experiments. (*P < 0.05).
Normal Urothelial Cell Primary Cell Huc, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
normal urothelial cell primary cell huc - by Bioz Stars, 2026-07
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( A ) Endogenous DPP4 mRNA levels were measured by using quantitative RT-PCR (right panel) and western blotting assays (left panel). Compared with non-tumorigenic urothelial primary cell HUC, two UC cells, J82 and RTCC-1, show high DPP4 mRNA and protein expression levels. ( B ) To explore biological functions of DPP4 in vitro, DPP4 knockdown is conducted by using short-hairpin RNA which successfully deplete DPP4 transcript level in J82 (right panel) and RTCC-1 (left panel) cells. ( C ) Depletion of DPP4 expression results in a significantly decreased cell viability (upper panel), migration (middle panel), and invasion (lower panel) in J82 and RTCC-1 cells. The quantified results are presented as means ± sd. Error bars indicate the standard error of the mean. Data represent mean values of three independent experiments. (*P < 0.05).

Journal: Oncotarget

Article Title: DPP4/CD26 overexpression in urothelial carcinoma confers an independent prognostic impact and correlates with intrinsic biological aggressiveness

doi: 10.18632/oncotarget.13820

Figure Lengend Snippet: ( A ) Endogenous DPP4 mRNA levels were measured by using quantitative RT-PCR (right panel) and western blotting assays (left panel). Compared with non-tumorigenic urothelial primary cell HUC, two UC cells, J82 and RTCC-1, show high DPP4 mRNA and protein expression levels. ( B ) To explore biological functions of DPP4 in vitro, DPP4 knockdown is conducted by using short-hairpin RNA which successfully deplete DPP4 transcript level in J82 (right panel) and RTCC-1 (left panel) cells. ( C ) Depletion of DPP4 expression results in a significantly decreased cell viability (upper panel), migration (middle panel), and invasion (lower panel) in J82 and RTCC-1 cells. The quantified results are presented as means ± sd. Error bars indicate the standard error of the mean. Data represent mean values of three independent experiments. (*P < 0.05).

Article Snippet: A normal urothelial cell primary cell, HUC (ScienCell Research Laboratories, San Diego, CA), was used as a control.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, In Vitro, Knockdown, shRNA, Migration